How to determine hepatitis B surface antigen

Hepatitis B surface antigen (HBsAg) is an important marker of hepatitis B virus infection, and its measurement is crucial for the diagnosis and treatment monitoring of hepatitis B. At present, the commonly used detection methods mainly include enzyme-linked immunosorbent assay (ELISA) and chemiluminescent immunoassay.

The ELISA method uses the principle of specific binding of antigens and antibodies to react with the hepatitis B surface antigen in the sample through the reaction of enzyme-labeled antibodies and determine the antigen content based on the degree of color development. This method is simple to operate and low in cost, but has limitations in sensitivity and specificity. The chemiluminescent immunoassay method is more advanced. It uses chemiluminescent substances to label antibodies and quantitatively analyzes hepatitis B surface antigen by detecting luminescence intensity. It has higher sensitivity and specificity, and is especially suitable for the detection of low-concentration antigens.

In terms of treatment, drugs that inhibit hepatitis B virus surface antigen include entecavir tablets, tenofovir alafenamide tablets, etc. These drugs inhibit viral replication through different mechanisms, thereby reducing antigen levels and are an important component of hepatitis B treatment. In summary, the accurate determination of hepatitis B surface antigen plays an irreplaceable role in the prevention, control and treatment of hepatitis B.